3-[(3aR-6aS)-6--chloro-5-(4-chlorophenyl)-7--methyl-2--4-6-trioxo-1-spiro[1-2-3a-6a-tetrahydropyrrolo[3-4-c]pyrrole-3-3--1H-indole]yl]propanamide has been researched along with Inflammation* in 1 studies
1 other study(ies) available for 3-[(3aR-6aS)-6--chloro-5-(4-chlorophenyl)-7--methyl-2--4-6-trioxo-1-spiro[1-2-3a-6a-tetrahydropyrrolo[3-4-c]pyrrole-3-3--1H-indole]yl]propanamide and Inflammation
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Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening.
This protocol describes microsphere-based protease assays for use in flow cytometry and high-throughput screening. This platform measures a loss of fluorescence from the surface of a microsphere due to the cleavage of an attached fluorescent protease substrate by a suitable protease enzyme. The assay format can be adapted to any site or protein-specific protease of interest and results can be measured in both real time and as endpoint fluorescence assays on a flow cytometer. Endpoint assays are easily adapted to microplate format for flow cytometry high-throughput analysis and inhibitor screening. Topics: Animals; Biotinylation; Flow Cytometry; Fluorescence Resonance Energy Transfer; Green Fluorescent Proteins; High-Throughput Screening Assays; Humans; Inflammation; Kinetics; Microspheres; Peptide Hydrolases; Peptides; Reproducibility of Results; Temperature | 2010 |